Journal: Journal of Virology

Article Title: Adenovirus Modulates Toll-Like Receptor 4 Signaling by Reprogramming ORP1L-VAP Protein Contacts for Cholesterol Transport from Endosomes to the Endoplasmic Reticulum

doi: 10.1128/JVI.01904-16

Figure Lengend Snippet: Reagents and kits used in this study

Article Snippet: Description of antibodies used in the study a table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 Reagent Company Catalog no. ALLN Calbiochem (Billerica, MA) 110044-82-1 Bio-Rad protein assay kit Bio-Rad (Hercules, CA) 500-0001 Cell fractionation kit Cell Biolabs AKR-171 HRS siRNA SMARTpool Dharmacon (Pittsburgh, PA) L-016835 ORP1L HRS siRNA SMARTpool Dharmacon M-008350 ORP5 siRNA SMARTpool Dharmacon L-009274 Rab7 siRNA SMARTpool Dharmacon L-010388 ECL detection reagent GE Life Sciences (Pittsburgh, PA) RPN2236 Percoll GE Life Sciences 17-0891-01 Amplex Red cholesterol assay kit Invitrogen (Grand Island, NY) {"type":"entrez-nucleotide","attrs":{"text":"A12216","term_id":"489486","term_text":"A12216"}} A12216 Lipofectamine Invitrogen 11668-019 Oligofectamine Invitrogen 12252-011 Bodipy 493/503 Invitrogen D3922 DAPI Invitrogen D1306 LipidTOX Invitrogen {"type":"entrez-nucleotide","attrs":{"text":"H34157","term_id":"979574","term_text":"H34157"}} H34157 SlowFade antifade kit Invitrogen S2828 Anti-GFP-agarose MBL (Woburn, MA) D153-8 Glutathione-agarose Pierce (Rockford, IL) 16100 AllStars negative-control siRNA Qiagen (Valencia, CA) 1027280 Cab-O-Sil Sigma (St. Louis, MO) 13760 Filipin Sigma F4767 Lipopolysaccharide (LPS) Sigma L5293 Poly- l -lysine Sigma P4707 Protein A Sepharose Sigma P3391 Open in a separate window Reagents and kits used in this study Cells and tissue culture.

Techniques: Cell Fractionation, Amplex Red Cholesterol Assay, Negative Control